美国布莱根妇女医院Yu Shrike Zhang团队的最新研究提出了细胞密集组织的生物材料极简光活化生物打印。2025年12月8日出版的《细胞》杂志发表了这项成果。
该课题组研究人员提出了一种用丙烯酸酯键修饰细胞膜的方法，使活细胞在生理密度下(高达~109个细胞mL-1)直接作为生物墨水，通过数字光处理展示纯细胞生物墨水的光活化生物打印。他们的细胞密集的生物墨水（CLINKs）迅速产生组织结构，密切模仿天然组织，具有很强的结构相关性和机器人功能。CLINKs的高细胞性和活性特性使其能够创建先进的生物模型，如连接的神经回路和完全由干细胞衍生的有节奏收缩的微型心脏，有效地捕获基本的原生行为。通过这种方法产生的植入物显示出与宿主融合的能力，从而促进再生。他们的CLINK技术在组织生物制造方面有着巨大的前景，为生物医学应用开辟了另一种途径。
研究人员表示，传统的基于水凝胶的生物打印方法往往存在细胞密度不足的问题，这可能会限制关键的细胞间相互作用并损害整体组织功能。
附：英文原文
Title: Biomaterial-minimalistic photoactivated bioprinting of cell-dense tissues
Author: Mian Wang, Wanlu Li, Jin Hao, Ling Cai, Xuan Mei, Regina Sanchez Flores, Penélope Cerón Castillo, Carlos Ezio Garciamendez-Mijares, Xuan Mu, Xiao Kuang, Xianbin Yu, Jugal Kishore Sahoo, Guosheng Tang, Zeyu Luo, Guillermo Wells, Zhongmin Liu, Alfredo Quiones-Hinojosa, Kevin Eggan, Shaorong Gao, Yu Shrike Zhang
Issue&Volume: 2025-12-08
Abstract: Conventional hydrogel-based bioprinting methods often suffer from insufficient cell densities, which may limit crucial cell-cell interactions and impair overall tissue functions. Here, we present an approach that modifies cell membranes with acrylate bonds, allowing living cells at physiological densities (up to ～109 cells mL1) to serve directly as bioinks, demonstrating photoactivated bioprinting through digital light processing using purely cellular bioinks. Our cell-dense bioinks (CLINKs) rapidly produce tissue constructs that closely mimic native tissues, characterized by strong structural relevancy and robust functionality. The high cellularity and living nature of CLINKs enable the creation of advanced biological models such as connected neural circuits and rhythmically contracting mini-hearts derived entirely from stem cells, effectively capturing essential native-like behaviors. Implants created through this method showcase the capacity to integrate with the host, thereby promoting regeneration. Our CLINK technology holds substantial promise in tissue biofabrication, opening alternative avenues for biomedical applications.
DOI: 10.1016/j.cell.2025.11.012
Source: https://www.cell.com/cell/abstract/S0092-8674(25)01308-X